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The concentration and quality of DNA extracted by the two types of sticks were the same. However, the results showed that significantly more PCR product was obtained by the DNA extracted from the EZ-D stick than from the Z-Dipstick (Figs. (Figs.1c 1 c and and1d). 1 d). The concentration and quality of DNA extracted by EZ-D are …
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The method combines a traditional CTAB DNA extraction and modified DNA clean-up procedure using silica-coated magnetic beads into a new platform that reduced …
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DNA isolation from filters was performed using two protocols to compare their efficiency (Tables S2 and S3). The first protocol is based on the use of 1% CTAB solution in the presence of 1 M NaCl ...
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EZ-D has been successfully used for DNA extraction from a variety of plant species and plant tissues and the DNA is suitable for a wide range of downstream …
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quantifying DNA based on PCR. qPCR tracks target concentration as a function of PCR cycle number in order to derive a quantitative estimate of the initial template concentration in a sample. As with conventional PCR, it uses a polymerase, dNTPs, and two primers designed to match sequences within a template.
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While soil testing is performed to predict the nutrient availability in soils, plant tissue analysis provides information on the nutrients taken up by the plants. The phosphorus concentration in plant tissue might be in deficiency range, sufficiency range, or excess range.
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DNA concentration of the extraction method ranged from 8.8 to 9.9 ... The concentration of NaCl varied with plant species in a range between 0.7 M to 6 M . In the present study, higher level of NaCl (1.5 M) in the extraction buffer further improved the quality of the extracted DNA. The high quality of obtained DNA could also be attributed …
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Agarose concentration (% w/v) DNA fragment range (kb) 0.3* 5–60: 0.5: 1–30: 0.7: 0.8–12: 1.0: 0.5–10: 1.2: 0.4–7: 1.5: 0.2–3: ... This will depend on the size of DNA being analyzed, the concentration of agarose in the gel, and the separation required. ... Plant tissue; Fungal material; DNA cleanup. Isopropanol precipitation of DNA.
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Accurate measurement of DNA concentration is important for DNA-based biological applications. DNA concentration is usually determined by the ultraviolet (UV) absorption, fluorescence staining, and diphenylamine reaction methods. However, the best method for quality assurance of measurements is unkno …
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Cite this article. Carter, S., Meyerson, M. & Getz, G. Accurate estimation of homologue-specific DNA concentration-ratios in cancer samples allows long-range haplotyping.
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where: C C C – Concentration of the nucleic acid in the sample.. A 260 A_{260} A 260 – The maximum absorbance as indicated by the spectrophotometric reading. This usually occurs at the wavelength of …
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The present work describes an inexpensive CTAB-based method with modifications for the extraction of high-quality genomic DNA from 19 different plant …
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Reliable measurement of DNA concentration and purity is important for many applications in molecular biology where accurate determination of DNA concentration is critical. ... If the ratio is out of the general acceptable range, it may indicate the presence of contaminants which absorb at 230 nm. ... Carbohydrates are a …
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The mismatch could have occurred because the DNA concentration is not the only determining factor of the resulting DNA band; in other words, the DNA concentration does not influence the resulting ...
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Accurate DNA quantification is key for many molecular biology workflows. Without precise knowledge of your sample's concentration, yield and purity, the success of downstream applications can be put at risk. This article describes the different methods available for quantifying DNA, highlighting their strengths, weaknesses and the essential …
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Microspore embryogenesis is a process that produces doubled haploids in tissue culture environments and is widely used in cereal plants. The efficient production of green regenerants requires stresses that could be sensed at the level of glycolysis, followed by the Krebs cycle and electron transfer chain. The latter can be affected by Cu(II) ion …
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DNA concentration and quality in plant extractions. (A) Concentration (ng/ μ L) ± 1 SE as detected by the Epoch Spectrophotometer System determined for seven replicates. Nucleic acid...
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DNA was successfully recovered from all of the samples. The E. hemionus was the species yielded a higher DNA amount, with an average of 71.12 ng/μl, ranging from 7.39 to 179 ng/μl. The E. africanus samples produced DNA concentrations between 9.90 and 257 ng/μl, with an average of 40.16 ng/μl.E. kiang produced an average of 24.97 …
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The A260/A280 provides insight regarding the type of nucleic acid present (dsDNA or RNA) as well as providing a rough indication of purity. Typically, protein contamination can be detected by a reduction of this ratio; RNA contamination can be detected by an increase …
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Recommended Agarose Gels for Electrophoretic Separation of DNA Fragments. Agarose gel, % Range of effective separation, bp Approximate positions of tracking dyes, bp* Bromophenol blue Xylene cyanol FF TBE buffer TAE buffer TBE buffer TAE buffer 0.5 2000-50000 750 1150 13000 16700
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A NanoDrop spectrophotometer is a common lab instrument that can measure the concentration of DNA, RNA, and protein in a 2-µL drop on a pedestal. ... The NanoDrop's Linear Dynamic Range. The linear dynamic range of the NanoDrop spectrophotometer varies depending on the model (see Table 2). Table 2.
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DNA concentration is generally calculated using Lambert–Beer law from spectrophotometric analysis of the absorption at 260 nm ... The measurement of DNA concentration at a lower range can be strongly affected by light scattering on dust particles present in the sample. This method for measuring concentration is relatively …
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The optimal values for 260 nm/230 nm ratio calculations are 2.0–2.2. 25 The guidance on DNA concentration values provided herein is based on a single amplicon in the sample; samples containing multiple amplicons (e.g., from multiplex PCR reactions) add to the total weight of the measured DNA and will provide false assumptions when …
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The method presented here yielded a high quantity and concentration of DNA from 1 g of plant tissue for other plants, including capsicum ... Recommendations established here could prove adaptable to extract high-quality nucleic acids from tissues from a range of difficult plant species. Acknowledgments. The authors wish to …
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DNA concentration can be determined by measuring the absorbance at 260 nm (A 260) in a spectrophotometer using a quartz cuvette. For greatest accuracy, readings should be …
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Abstract Environmental DNA (eDNA) methods are increasingly used to detect aquatic organisms. To optimize survey efficiency in natural environments, it is necessary to understand the seasonal change in eDNA concentrations of target species and identify the season and environmental conditions in which eDNA concentrations are highest. …
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DNA concentration and quality in plant extractions. (A) Concentration (ng/ μ L) ± 1 SE as detected by the Epoch Spectrophotometer System determined for seven replicates. ... The DNA size range ...
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The DNA concentration measurements made using the Nanodrop were ... steps may have resulted in loss of genomic DNA. A range of different size beads are used in the bead beating step of precipB which may aide in lysis of different cell types resulting in ... based analysis of fungal and bacterial DNA. Plant Mol Biol Rep. 2004; 22:71–81 ...
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Run the samples next to DNA standards of known concentration or use molecular mass markers (DNA Mass Ladders, Invitrogen, 10068-013 or 10496-016). To maintain constant background staining of the gel, include 0.5 μg/mL ethidium bromide in the running buffer. Use a UV light to photograph the gel. ...
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Transcript abundance of the eight genes was also measured at different inflorescence developmental stages of T. ravennae (i.e., 40, 80, 120, 160, and 200 cm in length). The average Ct values of ...
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DNA concentration is good if the isolated DNA has a concentration above 20, with the purity value of the DNA extract in the range of 1.8-2.1. In this study, the isolation results have differences in the value of purity, wherein in this study, the value of puri ty is above 2.1. This is in line with the research
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Within a certain range, the fluorescence intensity is proportional to the amount of binding dye, so the DNA concentration of samples can be calculated by comparison with a standard curve generated from reference solutions of known DNA concentration [7], [8]. The principle of the diphenylamine reaction method is that the …
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Calculate your DNA sample concentration and purity using Addgene's DNA Quantification Protocol.
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(A) Median and quartile concentration range of DNA yielded from tissue stored on silica since 2012, 2019, or 2022. Significance is denoted by a blue asterisk. (B, C) Median and quartile range of DNA purity from tissue stored for different durations, represented by A 260 /A 280 (B) and A 260 /A 230 (C) absorbance ratios.
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